Among immortal human cell lines, the Jurkat cell line is another mostly used cell lines besides HeLa cells.
To read more about HeLa cells, click the button below.
1. What are Jurkat cells?
Jurkat cells were isolated from the peripheral blood of a 14-year-old boy with T cell leukemia. They are suspended in a spherical form, ranging between 10 and 16 um in size. These are the most well-known T cell lines that can be used to investigate T cell leukemia, HIV, and malignancies.
2. Characteristics of Jurkat cells
Cell structure:
Shape | Spherical |
Diameter | 10 – 16 μm |
Cell culture:
Jurkat cells are grown in suspension in RPMI 1640 medium containing glutathione, high concentration of vitamins including vitamin B12, along with biotin, PABA, and Fetal Bovine Serum (FBS) [1]. The purpose of RPMI 1640 was to culture human leukemic cells.
3. Use in medical research
Jurkat cells are commonly used to research T cell leukemia, T cell signaling, RNA silencing, and the activation and latency of viruses like HIV. Jurkat cells are produced from T-cell leukemia and are the most valuable resource for studying this cancer.
Jurkat cells have the CD4 T-cell receptor (TCR), which makes them sensitive to HIV infection. Since this receptor is required for HIV to enter and infect, Jurkat cells are the best model to research the precise mechanism of HIV infection [2].
References:
Introducing NanoEntek latest automated cell counters
Cell types | Cell lines, primary cells |
Measuring range | 5x10E4 - 2x10E7 4x10E5 - 1x10E7 (optimal) |
Loading volume | 15 μL |
Staining reagent | Acridine orange / DAPI |
Cell types | Cell lines, primary cells |
Measuring range | 5x10E4 - 2x10E74x10E5 - 1x10E7 (optimal) |
Loading volume | 15 μL |
Staining reagent | Acridine orange / DAPI |
21 CFR Part 11 compliant | Yes |
NanoEntek also provides a live cell imaging system
Objective lens | 4x, 10x, 20x |
Channel type | Brightfield, GFP, RFP, DAPI |
Vessel type | Well plate, flask, dish, slide |
Incubator installation | Available |
Comments