What is DAPI?
DAPI is a fluorescent stain that bonds specifically to the A-T-rich parts of double-stranded DNA. Given that DAPI is not a cell-permeant solution, it only dyes dead or fixed cells as it cannot effectively enter the membranes of living, intact cells. A much higher concentration is required to stain live cells. DAPI is excited by ultraviolet (UV) light and emits a blue fluorescence.
Characteristics of DAPI
DAPI solution specifically stains dead cells, making it ideal for applications such as cell counting, apoptosis detection, and cell cycle analysis. Its staining capability relies on the integrity of the cell membrane, enabling the effective identification of dead cells during apoptosis, etc. Compared to similar dyes like Hoechst, DAPI exhibits a stronger fluorescence signal. However, DAPI is sensitive to light exposure, which can cause its fluorescence signal to fade more quickly. Additionally, it is pH-dependent and binds optimally to DNA at a neutral pH of 7.0.
Properties
Molecular weight : 277.32 g/mol
Excitation / emission peak : 359 nm / 457 nm
Permeability : X
NanoEntek provides automated cell counters using a DAPI staining solution.
Opmerkingen